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Method Comments Histopathology(Hematoxylin and eosin staining)(Warthin-Starry staining) Check for:lobular, vascular proliferationneutrophils and debrisbasophilic granular materialprotuberant endothelial cellsdarkly staining bacilli Blood culture lysis-centrifugation tubes, onto fresh chocolate and rabbit-blood agarsincubate in 5% CO2 at 35°C for 21 daysaccessible to many labs Culture of tissue biopsy experimental cocultivation of endothelial cells with homogenate Serology Centers for Disease Control indirect immunofluorescence antibody (IFA) test

Reprinted with permission from Koehler J. Recurrent bacterial infections: bartonella. Clinical Care Options for HIV 1995; 1(3):17-18.