- Moisten a Dacron swab with saline or tap water. An endocervical cytobrush can also be used to obtain cytologic material. Insert swab into the distal rectum, at least 2 cm from the anal verge.
- Rotate the swab while withdrawing through the anal canal.
- Smear the swab onto a glass slide and fix with spray fixative or by immersion in alcohol within 10 seconds of withdrawal.
- Stain fixed smear by routine Pap method for cytological interpretation.
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